Flow Cytometry Protocols (Methods in Molecular Biology)

Product Description
Review
From reviews of the first edition a well and precisely written collection of protocols which provide important information for both experienced and novice flow cytometry users. - FEBS Letters There are many people who would benefit from reading this book, from those currently using cytometry to molecular biologists interested in looking at DNA or RNA in the context of cell phenotype in mixed cell populations and those involved in diagnostic testing - Molecular Biotechnology

Product Description
This thoroughly revised and updated edition of a widely used practical guide to flow cytometry describes in step-by-step detail an array of time proven and cutting-edge techniques much needed in today's advanced laboratories. These readily reproducible methods deploy emerging flow cytometry technologies in many new applications, especially in the field of stem cells, functional genomics and proteomics, and microbiology. Here, the aspiring investigator will find methods for the characterization of stem/progenitor cells by monitoring the efflux of fluorescent dyes and the elucidation of signal transduction pathways using phospho-specific antibodies. There are also techniques for monitoring gene transfer and expression using fluorescent protein technology, high throughput screening for discovery of novel protein interactions, phenotypic and functional characterization of T cell subsets and precursors, and microbial flow cytometry, to highlight but some of the many useful procedures.


Table of Contents
Preface
Contributors
1 Flow cytometry : an introduction 1
2 Multiparameter flow cytometry of bacteria 33
3 Multiparameter data acquisition and analysis of leukocytes 45
4 Flow cytometric analysis of kinase signaling cascades 67
5 Cytokine flow cytometry 95
6 Use of cell-tracking dyes to determine proliferation precursor frequencies of antigen-specific T cells 109
7 Assessment of lymphocyte-mediated cytotoxicity using flow cytometry 125
8 Multiparametric analysis of apoptosis by flow and image cytometry 141
9 Detection and enrichment of haematopoietic stem cells by side population phenotype 161
10 Hematopoietic stem cell characterization by Hoechst 33342 and Rhodamine 123 staining 181
11 Phenotypic and functional analyses of CD34[superscript NEG] haematopoietic precursors from mobilized peripheral blood 201
12 Multiparameter flow cytometry of fluorescent protein reporters 219
13 Analysis of fluorescent protein expressing cells by flow cytometry 239
14 Integrative flow cytometric and microarray approaches for use in transcriptional profiling 259
15 Flow cytometric analysis of fluorescence resonance energy transfer : a tool for high-throughput screening of molecular interactions in living cells 281
16 Design of a fluorescence-activated cell sorting-based mammalian protein-protein interaction trap 293
17 Flow cytometric screening of yeast surface display libraries 311
18 Fluorescence resonance energy transfer-based HIV-1 virion fusion assay 333
19 Cell-cycle analysis of asynchronous populations 345
20 Solid tumor DNA content analysis 355
21 Concurrent flow cytometric analysis of DNA and RNA 371
22 Telomere length measurement by fluorescence in situ hybridization and flow cytometry 385
23 Small lasers in flow cytometry 399
24 Viable infections cell sorting in a BSL-3 facility 419
Index 425





# 434 pages
# Publisher: Humana Press; 2 edition (April 2004)
# Language: English
# ISBN-10: 1588292347
# ISBN-13: 978-1588292346

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